In quantum computing, a qubit or quantum bit (sometimes qbit) is the basic unit of quantum information: H
In particle physics, a hadron /ˈhædrɒn/ (Greek: ἁδρός, hadrós; “stout, thick”) is a subatomic composite particle made of two or more quarks held together by the strong force in a similar way as molecules are held together by the electromagnetic force. Most of the mass of ordinary matter comes from two hadrons: the proton and the neutron.
Hadrons are categorized into two families: baryons, made of an odd number of quarks – usually three quarks – and mesons, made of an even number of quarks—usually one quark and one antiquark. Protons and neutrons (which make the majority of the mass of an atom) are examples of baryons; pions are an example of a meson. “Exotic” hadrons, containing more than three valence quarks, have been discovered in recent years. A tetraquark state (an exotic meson), named the Z(4430)−, was discovered in 2007 by the Belle Collaboration and confirmed as a resonance in 2014 by the LHCb collaboration. Two pentaquark states (exotic baryons), named P+
c(4380) and P+
c(4450), were discovered in 2015 by the LHCb collaboration. There are several more exotic hadron candidates, and other colour-singlet quark combinations that may also exist.
Almost all “free” hadrons and antihadrons (meaning, in isolation and not bound within an atomic nucleus) are believed to be unstable and eventually decay (break down) into other particles. The only known exception relates to free protons, which are possibly stable, or at least, take immense amounts of time to decay (order of 1034+ years). Free neutrons are unstable and decay with a half-life of about 611 seconds. Their respective antiparticles are expected to follow the same pattern, but they are difficult to capture and study, because they immediately annihilate on contact with ordinary matter. “Bound” protons and neutrons, contained within an atomic nucleus, are generally considered stable. Experimentally, hadron physics is studied by colliding protons or nuclei of heavy elements such as lead or gold, and detecting the debris in the produced particle showers. In the natural environment, mesons such as pions are produced by the collisions of cosmic rays with the atmosphere.
Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Although one may divide microscopic anatomy into organology, the study of organs, histology, the study of tissues, and cytology, the study of cells, modern usage places these topics under the field of histology. In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. In the field of paleontology, the term paleohistology refers to the histology of fossil organisms.
In the 17th century the Italian Marcello Malpighi used microscopes to study tiny biological entities; some regard him as the founder of the fields of histology and microscopic pathology. Malpighi analyzed several parts of the organs of bats, frogs and other animals under the microscope. While studying the structure of the lung, Malpighi noticed its membranous alveoli and the hair-like connections between veins and arteries, which he named capillaries. His discovery established how the oxygen breathed in enters the blood stream and serves the body.
In the 19th century histology was an academic discipline in its own right. The French anatomist Xavier Bichat introduced the concept of tissue in anatomy in 1801 and the term “histology” (German: Histologie), coined to denote the “study of tissues”, first appeared in a book by Karl Meyer in 1819. Bichat described twenty-one human tissues, which can be subsumed under the four categories currently accepted by histologists. The usage of illustrations in histology, deemed as useless by Bichat, was promoted by Jean Cruveilhier.
In the early 1830s Purkynĕ invented a microtome with high precision.
During the 19th century many fixation techniques were developed by Adolph Hannover (solutions of chromates and chromic acid), Franz Schulze and Max Schultze (osmic acid), Alexander Butlerov (formaldehyde) and Benedikt Stilling (freezing).
Mounting techniques were developed by Rudolf Heidenhain (gum Arabic), Salomon Stricker (mixture of wax and oil), Andrew Pritchard (gum and isinglass) and Edwin Klebs (Canada balsam).
The 1906 Nobel Prize in Physiology or Medicine was awarded to histologists Camillo Golgi and Santiago Ramon y Cajal. They had conflicting interpretations of the neural structure of the brain based on differing interpretations of the same images. Ramón y Cajal won the prize for his correct theory, and Golgi for the silver-staining technique he invented to make it possible.
Animal tissue classification
There are four basic types of animal tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue. All animal tissues are considered to be subtypes of these four principal tissue types (for example, blood is classified as connective tissue, since the blood cells are suspended in an extracellular matrix, the plasma).
- Simple epithelium
- Simple squamous epithelium
- Simple cuboidal epithelium
- Simple columnar epithelium
- Pseudostratified columnar epithelium
- Stratified epithelium
- Stratified squamous epithelium
- Stratified cuboidal epithelium
- Stratified columnar epithelium
- Transitional epithelium
- Multicellular glands
- Simple epithelium
- Muscle tissue
- Smooth muscle
- Skeletal muscle
- Cardiac muscle
- Connective tissue
- General connective tissue
- Loose connective tissue
- Dense connective tissue
- Special connective tissue
- General connective tissue
- Nervous tissue
- Central nervous system
- Peripheral nervous system
- Special receptors
Plant tissue classification
For plants, the study of their tissues falls under the field of plant anatomy, with the following four main types:
- Dermal tissue
- Vascular tissue
- Ground tissue
- Meristematic tissue
Histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. It is an important part of anatomical pathology and surgical pathology, as accurate diagnosis of cancer and other diseases often requires histopathological examination of tissue samples. Trained physicians, frequently licensed pathologists, perform histopathological examination and provide diagnostic information based on their observations.
Humans (Homo sapiens) are a species of highly intelligent primates. They are the only extant members of the subtribe Hominina and—together with chimpanzees, gorillas, and orangutans—are part of the family Hominidae (the great apes, or hominids). Humans are terrestrial animals, characterized by their erect posture and bipedal locomotion; high manual dexterity and heavy tool use compared to other animals; open-ended and complex language use compared to other animal communications; larger, more complex brains than other primates; and highly advanced and organized societies.
Several early hominins used fire and occupied much of Eurasia. Early modern humans are thought to have diverged in Africa from an earlier hominin around 300,000 years ago, with the earliest fossil evidence of Homo sapiens also appearing around 300,000 years ago in Africa. Humans began to exhibit evidence of behavioral modernity at least by about 100,000–70,000 years ago (and possibly earlier). In several waves of migration, H. sapiens ventured out of Africa and populated most of the world. The spread of the large and increasing population of humans has profoundly affected the biosphere and millions of species worldwide. Among the key advantages that explain this evolutionary success is the presence of a larger, well-developed brain, which enables advanced abstract reasoning, language, problem solving, sociality, and culture through social learning. Humans use tools more frequently and effectively than any other animal: they are the only extant species to build fires, cook food, clothe themselves, and create and use numerous other technologies and arts.
Humans uniquely use systems of symbolic communication such as language and art to express themselves and exchange ideas, as well as to organize themselves into purposeful groups. Humans create complex social structures composed of many cooperating and competing groups, from families and kinship networks to political states. Social interactions between humans have established an extremely wide variety of values, social norms, and rituals, which together undergird human society. Curiosity and the human desire to understand and influence the environment and to explain and manipulate phenomena have motivated humanity’s development of science, philosophy, mythology, religion, and other fields of knowledge.
Though most of human existence has been sustained by hunting and gathering in band societies, many human societies transitioned to sedentary agriculture approximately 10,000 years ago, domesticating plants and animals, thus enabling the growth of civilization. These human societies subsequently expanded, establishing various forms of government and culture around the world, and unifying people within regions to form states and empires. The rapid advancement of scientific and medical understanding in the 19th and 20th centuries permitted the development of more efficient medical tools and healthier lifestyles, resulting in increased lifespans and causing the human population to rise exponentially. The global human population is about 7.8 billion in 2020.
The human genome is a complete set of nucleic acid sequences for humans, encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. These are usually treated separately as the nuclear genome, and the mitochondrial genome. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1% due to single-nucleotide variants and 0.6% when considering indels), these are considerably smaller than the differences between humans and their closest living relatives, the bonobos and chimpanzees (~1.1% fixed single-nucleotide variants and 4% when including indels).
The first human genome sequences were published in nearly complete draft form in February 2001 by the Human Genome Project and Celera Corporation. Completion of the Human Genome Project’s sequencing effort was announced in 2004 with the publication of a draft genome sequence, leaving just 341 gaps in the sequence, representing highly-repetitive and other DNA that could not be sequenced with the technology available at the time. The human genome was the first of all vertebrates to be sequenced to such near-completion, and as of 2018, the diploid genomes of over a million individual humans had been determined using next-generation sequencing. These data are used worldwide in biomedical science, anthropology, forensics and other branches of science. Such genomic studies have led to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.
Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.
Prior to the acquisition of the full genome sequence, estimates of the number of human genes ranged from 50,000 to 140,000 (with occasional vagueness about whether these estimates included non-protein coding genes). As genome sequence quality and the methods for identifying protein-coding genes improved, the count of recognized protein-coding genes dropped to 19,000-20,000. However, a fuller understanding of the role played by sequences that do not encode proteins, but instead express regulatory RNA, has raised the total number of genes to at least 46,831, plus another 2300 micro-RNA genes. By 2012, functional DNA elements that encode neither RNA nor proteins have been noted. and another 10% equivalent of human genome was found in a recent (2018) population survey. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA genes, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been determined.
In June 2016, scientists formally announced HGP-Write, a plan to synthesize the human genome.
Human Genome Project
The Human Genome Project (HGP) was an international scientific research project with the goal of determining the base pairs that make up human DNA, and of identifying and mapping all of the genes of the human genome from both a physical and a functional standpoint. It remains the world’s largest collaborative biological project. Planning started after the idea was picked up in 1984 by the US government, the project formally launched in 1990, and was declared complete on April 14, 2003.
Funding came from the US government through the National Institutes of Health (NIH) as well as numerous other groups from around the world. A parallel project was conducted outside the government by the Celera Corporation, or Celera Genomics, which was formally launched in 1998. Most of the government-sponsored sequencing was performed in twenty universities and research centres in the United States, the United Kingdom, Japan, France, Germany, Spain and China.
The Human Genome Project originally aimed to map the nucleotides contained in a human haploid reference genome (more than three billion). The “genome” of any given individual is unique; mapping the “human genome” involved sequencing a small number of individuals and then assembling these together to get a complete sequence for each chromosome. Therefore, the finished human genome is a mosaic, not representing any one individual.
State of completion
The project was not able to sequence all the DNA found in human cells. It sequenced only euchromatic regions of the genome, which make up 92.1% of the human genome. The other regions, called heterochromatic, are found in centromeres and telomeres, and were not sequenced under the project.
The Human Genome Project (HGP) was declared complete in April 2003. An initial rough draft of the human genome was available in June 2000 and by February 2001 a working draft had been completed and published followed by the final sequencing mapping of the human genome on April 14, 2003. Although this was reported to cover 99% of the euchromatic human genome with 99.99% accuracy, a major quality assessment of the human genome sequence was published on May 27, 2004 indicating over 92% of sampling exceeded 99.99% accuracy which was within the intended goal.
In March 2009, the Genome Reference Consortium (GRC) released a more accurate version of the human genome, but that still left more than 300 gaps, while 160 such gaps remained in 2015. Though in May 2020, the GRC reported 79 “unresolved” gaps, accounting for as much as 5% of the human genome, months later the application of new long-range sequencing techniques and a homozygous cell line in which both copies of each chromosome are identical led to the first telomere-to-telomere, truly complete sequence of a human chromosome, the X-chromosome. Work to complete the remaining chromosomes using the same approach is ongoing.
Applications and proposed benefits
The sequencing of the human genome holds benefits for many fields, from molecular medicine to human evolution. The Human Genome Project, through its sequencing of the DNA, can help us understand diseases including: genotyping of specific viruses to direct appropriate treatment; identification of mutations linked to different forms of cancer; the design of medication and more accurate prediction of their effects; advancement in forensic applied sciences; biofuels and other energy applications; agriculture, animal husbandry, bioprocessing; risk assessment; bioarcheology, anthropology and evolution. Another proposed benefit is the commercial development of genomics research related to DNA based products, a multibillion-dollar industry.
The sequence of the DNA is stored in databases available to anyone on the Internet. The U.S. National Center for Biotechnology Information (and sister organizations in Europe and Japan) house the gene sequence in a database known as GenBank, along with sequences of known and hypothetical genes and proteins. Other organizations, such as the UCSC Genome Browser at the University of California, Santa Cruz, and Ensembl present additional data and annotation and powerful tools for visualizing and searching it. Computer programs have been developed to analyze the data because the data itself is difficult to interpret without such programs. Generally speaking, advances in genome sequencing technology have followed Moore’s Law, a concept from computer science which states that integrated circuits can increase in complexity at an exponential rate. This means that the speeds at which whole genomes can be sequenced can increase at a similar rate, as was seen during the development of the above-mentioned Human Genome Project.